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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
26/09/2013 |
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Data da última atualização: |
19/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVEIRA, D. G.; LINO, L. S. M.; SOUZA, A. da S.; SOUZA, F. V. D. |
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Afiliação: |
DANIELA GARCIA SILVEIRA, IF Baiano; LUCIMEIRE SOUZA MORAIS LINO, Capes; ANTONIO DA SILVA SOUZA, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Somatic Embryogenesis of Neoglaziovia variegata (Arruda) Mez, an Important Source of Fiber from Native Brazilian Bromeliads. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Brazilian Archives of Biology and Technology, v.56 n.4: pp. 547-555, July/Aug. 2013. |
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ISSN: |
1516-8913 |
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Idioma: |
Português |
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Conteúdo: |
Neoglaziovia variegata (Arruda) Mez, a Bromeliad endemic to the Brazilian Caatinga and source of fiber for many different products, is considered an endangered species. Leaf and stem segments were cultivated on Murashige and Skoog (MS) medium with combinations of 2,4- dichlorophenoxyacetic acid (2,4-D) (0, 9, 18 and 27 µM) and glutamine (0, 50, 100, 150, 200 mg L-1) for the induction of embryogenic calli. Leaf segments did not present a favorable response for the combinations analyzed. The best response for embryogenic calli frequency with the best distribution of somatic embryos on the surface and better conversion rate was obtained using the stems explants in the medium containing 18 µM 2,4-D and 100 mg L-1 glutamine. The embryogenic calli obtained from the different induction media were transferred to MS medium supplemented with 30 g.L-1 sucrose, 2.4 g.L-1 Phytagel®, 0.53 µM ?-naphthalene acetic acid (NAA) and 0.88 µM of 6-benzylaminopurine (BAP). The regenerated plants presented normal growth; however, many embryos did not fully develop. |
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Thesagro: |
Bromélia; Bromeliaceae; Cultura de meristema. |
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Thesaurus Nal: |
Glutamine; Tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01789naa a2200229 a 4500
001 1967207
005 2023-05-19
008 2013 bl uuuu u00u1 u #d
022 $a1516-8913
100 1 $aSILVEIRA, D. G.
245 $aSomatic Embryogenesis of Neoglaziovia variegata (Arruda) Mez, an Important Source of Fiber from Native Brazilian Bromeliads.$h[electronic resource]
260 $c2013
520 $aNeoglaziovia variegata (Arruda) Mez, a Bromeliad endemic to the Brazilian Caatinga and source of fiber for many different products, is considered an endangered species. Leaf and stem segments were cultivated on Murashige and Skoog (MS) medium with combinations of 2,4- dichlorophenoxyacetic acid (2,4-D) (0, 9, 18 and 27 µM) and glutamine (0, 50, 100, 150, 200 mg L-1) for the induction of embryogenic calli. Leaf segments did not present a favorable response for the combinations analyzed. The best response for embryogenic calli frequency with the best distribution of somatic embryos on the surface and better conversion rate was obtained using the stems explants in the medium containing 18 µM 2,4-D and 100 mg L-1 glutamine. The embryogenic calli obtained from the different induction media were transferred to MS medium supplemented with 30 g.L-1 sucrose, 2.4 g.L-1 Phytagel®, 0.53 µM ?-naphthalene acetic acid (NAA) and 0.88 µM of 6-benzylaminopurine (BAP). The regenerated plants presented normal growth; however, many embryos did not fully develop.
650 $aGlutamine
650 $aTissue culture
650 $aBromélia
650 $aBromeliaceae
650 $aCultura de meristema
700 1 $aLINO, L. S. M.
700 1 $aSOUZA, A. da S.
700 1 $aSOUZA, F. V. D.
773 $tBrazilian Archives of Biology and Technology$gv.56 n.4: pp. 547-555, July/Aug. 2013.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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